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Author: Daryl S. Henderson

Drosophila Cytogenetics Protocols

Original price was: Rs.9,389.Current price is: Rs.7,511.

In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls.

Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators.

1 in stock

Additional information

Weight 1.500 kg
Author

Daryl S. Henderson

ISBN

9781588290502

Publisher

Humana Press

Year Published

2003

Dimensions

15.50 x 2.54 x 24.00 cm

Language

English

Condition

New

Number of Pages

480

Description

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

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