This book provides hands-on instruction of various methodologies used for membrane transporter research. It includes traditional transporter research methods and protocols, such as molecular cloning, reconstitution, and kinetics. Various techniques that are commonly used for transporter studies are introduced, including confocal microscopy, electropysiological methods, and NMR. It also embraces some emerging fields and methodologies, such as pharmacogenomics, bioinformatics and the use of microarray technology. Bioinformatics has become an indispensable field in biomedical, and specifically, transporter research. Commonly used bioinformatics methods, such as tools and protocols for sequence analysis and motif studies, are provided here to assist research in membrane transporters. The development of methodologies and experiment design procedures for microarray studies are provided making this an invaluable tool for biomedical researchers in various fields who are interested in membrane transporters, including biochemists, molecular biologists, geneticists, physiologists, bioinformaticians, pharmaceutical scientists, and clinical researchers.